3v4r | pdb_00003v4r

Crystal structure of a UvrB dimer-DNA complex

spinqualitylabelsall models 3v4r, resolution 3.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3v4r is a 4 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ADP Gene: BSU35170, dinA, uvr, uvrB (Bacillus subtilis) Activity: Type III site-specific deoxyribonuclease, with EC number 3.1.21.5 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed UvrB has a central role in the highly conserved UvrABC pathway functioning not only as a damage recognition element but also as an essential component of the lesion tracking machinery. While it has been recently confirmed that the tracking assembly comprises a UvrA(2)B(2) heterotetramer, the configurations of the damage engagement and UvrB-DNA handover complexes remain obscure. Here, we present the first crystal structure of a UvrB dimer whose biological significance has been verified using both chemical cross-linking and electron paramagnetic resonance spectroscopy. We demonstrate that this dimeric species stably associates with UvrA and forms a UvrA(2)B(2)-DNA complex. Our studies also illustrate how signals are transduced between the ATP and DNA binding sites to generate the helicase activity pivotal to handover and formation of the UvrB(2)-DNA complex, providing key insights into the configurations of these important repair intermediates. Crystal structure of the UvrB dimer: insights into the nature and functioning of the UvrAB damage engagement and UvrB-DNA complexes.,Webster MP, Jukes R, Zamfir VS, Kay CW, Bagneris C, Barrett T Nucleic Acids Res. 2012 Sep 1;40(17):8743-8758. Epub 2012 Jun 30. PMID:22753105 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also UvrABC

Proteopedia Page Contributors and Editors (what is this?)

OCA