Structural highlights
Publication Abstract from PubMedThe crystal structure of the "ene" nicotinamide-dependent cyclohexenone reductase (NCR) from Zymomonas mobilis (PDB ID: 4A3U) has been determined in complex with acetate ion, FMN, and nicotinamide, to a resolution of 1.95 A. To study the activity and enantioselectivity of this enzyme in the bioreduction of activated alpha,beta-unsaturated alkenes, the rational design methods site- and loop-directed mutagenesis were applied. Based on a multiple sequence alignment of various members of the Old Yellow Enzyme family, eight single-residue variants were generated and investigated in asymmetric bioreduction. Furthermore, a structural alignment of various ene reductases predicted four surface loop regions that are located near the entrance of the active site. Four NCR loop variants, derived from loop-swapping experiments with OYE1 from Saccharomyces pastorianus, were analysed for bioreduction. The three enzyme variants, P245Q, D337Y and F314Y, displayed increased activity compared to wild-type NCR towards the set of substrates tested. The active-site mutation Y177A demonstrated a clear influence on the enantioselectivity. The loop-swapping variants retained reduction efficiency, but demonstrated decreased enzyme activity compared with the wild-type NCR ene reductase enzyme. Crystal Structure Determination and Mutagenesis Analysis of the Ene Reductase NCR.,Reich S, Hoeffken HW, Rosche B, Nestl BM, Hauer B Chembiochem. 2012 Oct 2. doi: 10.1002/cbic.201200404. PMID:23033175[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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