3zc0 | pdb_00003zc0

Structure of AfC3PO - duplex RNA complex

spinqualitylabelsall models 3zc0, resolution 2.98Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3zc0 is a 15 chain structure with sequence from Arcfl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, MG Related: 3zc1 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Oligomeric complexes of Trax and Translin proteins, known as C3POs, participate in several eukaryotic nucleic acid metabolism pathways, including RNA interference and tRNA processing. In RNA interference in humans and Drosophila, C3PO activates the RNA-induced silencing complex (RISC) by removing the passenger strand of the small interfering RNA precursor duplex, using nuclease activity present in Trax. How C3POs engage with nucleic acid substrates is unknown. Here we identify a single protein from Archaeoglobus fulgidus that assembles into an octamer highly similar to human C3PO. The structure in complex with duplex RNA reveals that the octamer entirely encapsulates a single 13-base-pair RNA duplex inside a large inner cavity. Trax-like-subunit catalytic sites target opposite strands of the duplex for cleavage separated by 7 base pairs. The structure provides insight into the mechanism of RNA recognition and cleavage by an archaeal C3PO-like complex. Structural basis for duplex RNA recognition and cleavage by Archaeoglobus fulgidus C3PO.,Parizotto EA, Lowe ED, Parker JS Nat Struct Mol Biol. 2013 Jan 27. doi: 10.1038/nsmb.2487. PMID:23353787 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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