4g6d | pdb_00004g6d

G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex

spinqualitylabelsall models 4g6d, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4g6d is a 2 chain structure with sequence from Staphylococcus aureus subsp. aureus nctc 8325 and Staphylococcus phage g1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: rpoD, plaC, sigA, SAOUHSC_01662 (Staphylococcus aureus subsp. aureus NCTC 8325) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Phage G1 gp67 is a 23 kDa protein that binds to the Staphylococcus aureus (Sau) RNA polymerase (RNAP) sigma(A) subunit and blocks cell growth by inhibiting transcription. We show that gp67 has little to no effect on transcription from most promoters but is a potent inhibitor of ribosomal RNA transcription. A 2.0-A-resolution crystal structure of the complex between gp67 and Sau sigma(A) domain 4 (sigma(A)(4)) explains how gp67 joins the RNAP promoter complex through sigma(A)(4) without significantly affecting sigma(A)(4) function. Our results indicate that gp67 forms a complex with RNAP at most, if not all, sigma(A)-dependent promoters, but selectively inhibits promoters that depend on an interaction between upstream DNA and the RNAP alpha-subunit C-terminal domain (alphaCTD). Thus, we reveal a promoter-specific transcription inhibition mechanism by which gp67 interacts with the RNAP promoter complex through one subunit (sigma(A)), and selectively affects the function of another subunit (alphaCTD) depending on promoter usage. Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.,Osmundson J, Montero-Diez C, Westblade LF, Hochschild A, Darst SA Cell. 2012 Nov 21;151(5):1005-16. doi: 10.1016/j.cell.2012.10.034. PMID:23178120 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Sigma factor

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