4w4q | pdb_00004w4q

Glucose isomerase structure determined by serial femtosecond crystallography at SACLA

spinqualitylabelsall models 4w4q, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4w4q is a 1 chain structure with sequence from Streptomyces rubiginosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA Activity: Xylose isomerase, with EC number 5.3.1.5 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 A. Grease matrix as a versatile carrier of proteins for serial crystallography.,Sugahara M, Mizohata E, Nango E, Suzuki M, Tanaka T, Masuda T, Tanaka R, Shimamura T, Tanaka Y, Suno C, Ihara K, Pan D, Kakinouchi K, Sugiyama S, Murata M, Inoue T, Tono K, Song C, Park J, Kameshima T, Hatsui T, Joti Y, Yabashi M, Iwata S Nat Methods. 2014 Nov 10. doi: 10.1038/nmeth.3172. PMID:25384243 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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