3sd3 | pdb_00003sd3

The structure of the tetrahydrofolate riboswitch containing a U25C mutation

spinqualitylabelsall models 3sd3, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3sd3 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FOZ, IRI Related: 3sd1 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Transport and biosynthesis of folate and its derivatives are frequently controlled by the tetrahydrofolate (THF) riboswitch in Firmicutes. We have solved the crystal structure of the THF riboswitch aptamer in complex with folinic acid, a THF analog. Uniquely, this structure reveals two molecules of folinic acid binding to a single structured domain. These two sites interact with ligand in a similar fashion, primarily through recognition of the reduced pterin moiety. 7-deazaguanine, a soluble analog of guanine, binds the riboswitch with nearly the same affinity as its natural effector. However, 7-deazaguanine effects transcriptional termination to a substantially lesser degree than folinic acid, suggesting that the cellular guanine pool does not act upon the THF riboswitch. Under physiological conditions the ligands display strong cooperative binding, with one of the two sites playing a greater role in eliciting the regulatory response, which suggests that the second site may play another functional role. The Structure of a Tetrahydrofolate-Sensing Riboswitch Reveals Two Ligand Binding Sites in a Single Aptamer.,Trausch JJ, Ceres P, Reyes FE, Batey RT Structure. 2011 Sep 7. PMID:21906956 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Riboswitch

Proteopedia Page Contributors and Editors (what is this?)

OCA