2xns | pdb_00002xns

CRYSTAL STRUCTURE OF HUMAN G ALPHA I1 BOUND TO A DESIGNED HELICAL PEPTIDE DERIVED FROM THE GOLOCO MOTIF OF RGS14

spinqualitylabelsall models 2xns, resolution 3.41Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2xns is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GDP, SO4, SRT Related: 1kjy, 1y3a, 2gtp, 2ik8 Activity: Heterotrimeric G-protein GTPase, with EC number 3.6.5.1 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The de novo design of protein-binding peptides is challenging because it requires the identification of both a sequence and a backbone conformation favorable for binding. We used a computational strategy that iterates between structure and sequence optimization to redesign the C-terminal portion of the RGS14 GoLoco motif peptide so that it adopts a new conformation when bound to Galpha(i1). An X-ray crystal structure of the redesigned complex closely matches the computational model, with a backbone root-mean-square deviation of 1.1 A. Computational design of the sequence and structure of a protein-binding peptide.,Sammond DW, Bosch DE, Butterfoss GL, Purbeck C, Machius M, Siderovski DP, Kuhlman B J Am Chem Soc. 2011 Mar 30;133(12):4190-2. Epub 2011 Mar 9. PMID:21388199 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Guanine nucleotide-binding protein

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