2exq | pdb_00002exq

STRUCTURAL SUPERPOSITION OF MAB 806- EGFR PEPTIDE COMPLEX WITH EGFR HYPOTHETICAL EXTENDED MONOMER

spinqualitylabelsall models 2exq Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, PDBsum Publication Abstract from PubMed In this work, we combined computational protein-protein docking with computational and experimental mutagenesis to predict the structure of the complex formed by monoclonal antibody 806 (mAb 806) and the epidermal growth factor receptor (EGFR). We docked mAb 806, an antitumor antibody, to its epitope of EGFR residues 287-302. Potential mAb 806-EGFR orientations were generated, and computational mutagenesis was used to filter them according to their agreement with experimental mutagenesis data. Further computational mutagenesis suggested additional mutations, which were tested to arrive at a final structure that was most consistent with experimental mutagenesis data. We propose that this is the EGFR-mAb 806 structure, in which mAb 806 binds to an untethered form of the receptor, consistent with published experimental results. The steric hindrance created by the antibody near the EGFR dimer interface interferes with receptor dimerization, and we postulate this as the structural origin for the antitumor effect of mAb 806. Structural model of the mAb 806-EGFR complex using computational docking followed by computational and experimental mutagenesis.,Sivasubramanian A, Chao G, Pressler HM, Wittrup KD, Gray JJ Structure. 2006 Mar;14(3):401-14. PMID:16531225 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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