2y8y | pdb_00002y8y

STRUCTURE B OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 19 NT RNA

spinqualitylabelsall models 2y8y, resolution 1.44Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2y8y is a 2 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1wj9, 2y8w, 2y9h Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Clustered regularly interspaced short palindromic repeat (CRISPR) chromosomal loci found in prokaryotes provide an adaptive immune system against bacteriophages and plasmids. CRISPR-specific endoRNases produce short RNA molecules (crRNAs) from CRISPR transcripts, which harbor sequences complementary to invasive nucleic acid elements and ensure their selective targeting by CRISPR-associated (Cas) proteins. The extreme sequence divergence of CRISPR-specific endoRNases and their RNA substrates has obscured homology-based comparison of RNA recognition and cleavage mechanisms. Here, we show that Cse3 type CRISPR-specific endoRNases bind a hairpin structure and residues downstream of the cleavage site within the repetitive segment of cognate CRISPR RNA. Cocrystal structures of Cse3-RNA complexes reveal an RNA-induced conformational change in the enzyme active site that aligns the RNA strand for site-specific cleavage. These studies provide insight into a catalytically essential RNA recognition mechanism by a large class of CRISPR-related endoRNases. An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3.,Sashital DG, Jinek M, Doudna JA Nat Struct Mol Biol. 2011 Jun;18(6):680-7. Epub 2011 May 15. PMID:21572442 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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