3pvb | pdb_00003pvb

Crystal structure of (73-244)RIa:C holoenzyme of cAMP-dependent Protein kinase

spinqualitylabelsall models 3pvb, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3pvb is a 2 chain structure with sequence from Bos taurus and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ANP, GOL, MN NonStd Res: SEP, TPO Related: 1rgs, 2qcs Gene: Prkaca, Pkaca (Mus musculus), PRKAR1A (Bos taurus) Activity: cAMP-dependent protein kinase, with EC number 2.7.11.11 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed PKA holoenzymes containing two catalytic (C) subunits and a regulatory (R) subunit dimer are activated cooperatively by cAMP. While cooperativity involves the two tandem cAMP binding domains in each R-subunit, additional cooperativity is associated with the tetramer. Of critical importance is the flexible linker in R that contains an inhibitor site (IS). While the IS becomes ordered in the R:C heterodimer, the overall conformation of the tetramer is mediated largely by the N-Linker that connects the D/D domain to the IS. To understand how the N-Linker contributes to assembly of tetrameric holoenzymes, we engineered a monomeric RIalpha that contains most of the N-Linker, RIalpha(73-244), and crystallized a holoenzyme complex. Part of the N-linker is now ordered by interactions with a symmetry-related dimer. This complex of two symmetry-related dimers forms a tetramer that reveals novel mechanisms for allosteric regulation and has many features associated with full-length holoenzyme. A model of the tetrameric holoenzyme, based on this structure, is consistent with previous small angle X-ray and neutron scattering data, and is validated with new SAXS data and with an RIalpha mutation localized to a novel interface unique to the tetramer. Realizing the allosteric potential of the tetrameric protein kinase A RIalpha holoenzyme.,Boettcher AJ, Wu J, Kim C, Yang J, Bruystens J, Cheung N, Pennypacker JK, Blumenthal DA, Kornev AP, Taylor SS Structure. 2011 Feb 9;19(2):265-76. PMID:21300294 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CAMP-dependent protein kinase

Proteopedia Page Contributors and Editors (what is this?)

OCA