3wfz | pdb_00003wfz

Crystal structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase C236Y Mutant

spinqualitylabelsall models 3wfz, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wfz is a 4 chain structure with sequence from Bifidobacterium longum subsp. longum jcm 1217. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NDG Related: 2zuu Gene: BLLJ_1623, lnpA (Bifidobacterium longum subsp. longum JCM 1217) Activity: 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase, with EC number 2.4.1.211 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Galacto-N-biose/lacto-N-biose I phosphorylase (GLNBP) is the key enzyme in the enzymatic production of lacto-N-biose I. For the purpose of industrial use, we improved the thermostability of GLNBP by evolutionary engineering in which five substitutions in the amino acid sequence were selected from a random mutagenesis GLNBP library constructed using error-prone polymerase chain reaction. Among them, C236Y and D576V mutants showed considerably improved thermostability. Structural analysis of C236Y revealed that the hydroxyl group of Tyr236 forms a hydrogen bond with the carboxyl group of E319. The C236Y and D576V mutations together contributed to the thermostability. The C236Y/D576V mutant exhibited 20 degrees C higher thermostability than the wild type. Directed evolution to enhance thermostability of galacto-N-biose/lacto-N-biose I phosphorylase.,Koyama Y, Hidaka M, Nishimoto M, Kitaoka M Protein Eng Des Sel. 2013 Sep 24. PMID:24065834 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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