3zd7 | pdb_00003zd7

Snapshot 3 of RIG-I scanning on RNA duplex

spinqualitylabelsall models 3zd7, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3zd7 is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ADP, MG, ZN Related: 3zd6 Activity: RNA helicase, with EC number 3.6.4.13 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Retinoic acid-inducible gene-I (RIG-I) is an intracellular RNA sensor that activates the innate immune machinery in response to infection by RNA viruses. Here, we report the crystal structure of distinct conformations of a RIG-I:dsRNA complex, which shows that HEL2i-mediated scanning allows RIG-I to sense the length of RNA targets. To understand the implications of HEL2i scanning for catalytic activity and signalling by RIG-I, we examined its ATPase activity when stimulated by duplex RNAs of varying lengths and 5' composition. We identified a minimal RNA duplex that binds one RIG-I molecule, stimulates robust ATPase activity, and elicits a RIG-I-mediated interferon response in cells. Our results reveal that the minimal functional unit of the RIG-I:RNA complex is a monomer that binds at the terminus of a duplex RNA substrate. This behaviour is markedly different from the RIG-I paralog melanoma differentiation-associated gene 5 (MDA5), which forms cooperative filaments. Defining the functional determinants for RNA surveillance by RIG-I.,Kohlway A, Luo D, Rawling DC, Ding SC, Pyle AM EMBO Rep. 2013 Jul 30. doi: 10.1038/embor.2013.108. PMID:23897087 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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