4e68 | pdb_00004e68

Unphosphorylated STAT3B core protein binding to dsDNA

spinqualitylabelsall models 4e68, resolution 2.58Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4e68 is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1bg1 Gene: Aprf, Stat3, STAT3B (Mus musculus) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The STAT3 transcription factor plays a central role in a wide range of cancer types where it is over-expressed. Previously, phosphorylation of this protein was thought to be a prerequisite for direct binding to DNA. However, we have now shown complete binding of a purified unphosphorylated STAT3 (uSTAT3) core directly to M67 DNA, the high affinity STAT3 target DNA sequence, by a protein electrophoretic mobility shift assay (PEMSA). Binding to M67 DNA was inhibited by addition of increasing concentrations of a phosphotyrosyl peptide. X-ray crystallography demonstrates one mode of binding that is similar to that known for the STAT3 core phosphorylated at Y705. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: pSTAT3betatcandpSTAT3betatcbindbymolecular sieving(View interaction). Observation of unphosphorylated STAT3 core protein binding to target dsDNA by PEMSA and X-ray crystallography.,Nkansah E, Shah R, Collie GW, Parkinson GN, Palmer J, Rahman KM, Bui TT, Drake AF, Husby J, Neidle S, Zinzalla G, Thurston DE, Wilderspin AF FEBS Lett. 2013 Feb 19. pii: S0014-5793(13)00110-5. doi:, 10.1016/j.febslet.2013.01.065. PMID:23434585 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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