4e1u | pdb_00004e1u

[Ru(bpy)2 dppz]2+ bound to DNA

spinqualitylabelsall models 4e1u, resolution 0.92Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4e1u is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BA, R1Z Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed DNA mismatches represent a novel target in the development of diagnostics and therapeutics for cancer, because deficiencies in DNA mismatch repair are implicated in cancers, and cells that are repair-deficient show a high frequency of mismatches. Metal complexes with bulky intercalating ligands serve as probes for DNA mismatches. Here, we report the high-resolution (0.92 A) crystal structure of the ruthenium 'light switch' complex Delta-[Ru(bpy)(2)dppz](2+) (bpy = 2,2'-bipyridine and dppz = dipyridophenazine), which is known to show luminescence on binding to duplex DNA, bound to both mismatched and well-matched sites in the oligonucleotide 5'-(dCGGAAATTACCG)(2)-3' (underline denotes AA mismatches). Two crystallographically independent views reveal that the complex binds mismatches through metalloinsertion, ejecting both mispaired adenosines. Additional ruthenium complexes are intercalated at well-matched sites, creating an array of complexes in the minor groove stabilized by stacking interactions between bpy ligands and extruded adenosines. This structure attests to the generality of metalloinsertion and metallointercalation as DNA binding modes. Crystal structure of Delta-[Ru(bpy)(2)dppz](2)(+) bound to mismatched DNA reveals side-by-side metalloinsertion and intercalation.,Song H, Kaiser JT, Barton JK Nat Chem. 2012 Jun 10;4(8):615-20. doi: 10.1038/nchem.1375. PMID:22824892 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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