4ilr | pdb_00004ilr

Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6

spinqualitylabelsall models 4ilr, resolution 3.09Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ilr is a 1 chain structure with sequence from Sulfolobus solfataricus p2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3pkm, 4ill, 4ilm Gene: cas6b, SSO2004 (Sulfolobus solfataricus P2) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Clustered regularly interspaced short palindromic repeats (CRISPRs) confer adaptive immunity to prokaryotes through a small RNA-mediated mechanism. Specific endoribonucleases are required by all CRISPR-bearing organisms to process CRISPR RNAs into small RNA that serve as guides for defensive effector complexes. The molecular mechanism of how the endoribonucleases process the class of CRISPR RNA containing no predicted secondary structural features remains largely elusive. Here, we report cocrystal structures of a processing endoribonuclease bound with a noncleavable RNA substrate and its product-like fragment derived from a nonpalindramic repeat. The enzyme stabilizes a short RNA stem-loop structure near the cleavage site and cleaves the phosphodiester bond using an active site comprised of arginine and lysine residues. The distinct RNA binding and cleavage mechanisms underline the diversity in CRISPR RNA processing. Recognition and Cleavage of a Nonstructured CRISPR RNA by Its Processing Endoribonuclease Cas6.,Shao Y, Li H Structure. 2013 Feb 27. pii: S0969-2126(13)00017-8. doi:, 10.1016/j.str.2013.01.010. PMID:23454186 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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