4iqj | pdb_00004iqj

Structure of PolIIIalpha-Tauc-DNA complex suggests an atomic model of the replisome

spinqualitylabelsall models 4iqj, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4iqj is a 16 chain structure with sequence from Thermus aquaticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DOC, MG, ZN Related: 2hpi, 3e0d Gene: dnaE (Thermus aquaticus) Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The C-terminal domain (CTD) of the tau subunit of the clamp loader (tauc) binds to both the DnaB helicase and the DNA polymerase III alpha subunit (PolIIIalpha), and determines their relative positions and orientations on the leading and lagging strands. Here, we present a 3.2 A resolution structure of Thermus aquaticus PolIIIalpha in complex with tauc and a DNA substrate. The structure reveals that the CTD of tauc interacts with the CTD of PolIIIalpha through its C-terminal helix and the adjacent loop. Additionally, in this complex PolIIIalpha displays an open conformation that includes the reorientations of the oligonucleotide-binding fold and the thumb domain, which may be an indirect result of crystal packing due to the presence of the tauc. Nevertheless, the position of the tauc on PolIIIalpha allows us to suggest an approximate model for how the PolIIIalpha is oriented and positioned on the DnaB helicase. Structure of the PolIIIalpha-tauc-DNA complex suggests an atomic model of the replisome.,Liu B, Lin J, Steitz TA Structure. 2013 Apr 2;21(4):658-64. doi: 10.1016/j.str.2013.02.002. Epub 2013 Mar, 7. PMID:23478062 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase

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