4lct | pdb_00004lct

Crystal Structure and Versatile Functional Roles of the COP9 Signalosome Subunit 1

spinqualitylabelsall models 4lct, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4lct is a 4 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: SO4 Gene: CSN1, COP11, FUS6, At3g61140, T20K12.40 (Arabidopsis thaliana) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The constitutive photomorphogenesis 9 (COP9) signalosome (CSN) plays key roles in many biological processes, such as repression of photomorphogenesis in plants and protein subcellular localization, DNA-damage response, and NF-kappaB activation in mammals. It is an evolutionarily conserved eight-protein complex with subunits CSN1 to CSN8 named following the descending order of molecular weights. Here, we report the crystal structure of the largest CSN subunit, CSN1 from Arabidopsis thaliana (atCSN1), which belongs to the Proteasome, COP9 signalosome, Initiation factor 3 (PCI) domain containing CSN subunit family, at 2.7 A resolution. In contrast to previous predictions and distinct from the PCI-containing 26S proteasome regulatory particle subunit Rpn6 structure, the atCSN1 structure reveals an overall globular fold, with four domains consisting of helical repeat-I, linker helix, helical repeat-II, and the C-terminal PCI domain. Our small-angle X-ray scattering envelope of the CSN1-CSN7 complex agrees with the EM structure of the CSN alone (apo-CSN) and suggests that the PCI end of each molecule may mediate the interaction. Fitting of the CSN1 structure into the CSN-Skp1-Cul1-Fbox (SCF) EM structure shows that the PCI domain of CSN1 situates at the hub of the CSN for interaction with several other subunits whereas the linker helix and helical repeat-II of CSN1 contacts SCF using a conserved surface patch. Furthermore, we show that, in human, the C-terminal tail of CSN1, a segment not included in our crystal structure, interacts with IkappaBalpha in the NF-kappaB pathway. Therefore, the CSN complex uses multiple mechanisms to hinder NF-kappaB activation, a principle likely to hold true for its regulation of many other targets and pathways. Crystal structure and versatile functional roles of the COP9 signalosome subunit 1.,Lee JH, Yi L, Li J, Schweitzer K, Borgmann M, Naumann M, Wu H Proc Natl Acad Sci U S A. 2013 Jul 1. PMID:23818606 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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