2lpb | pdb_00002lpb

Structure of the complex of the central activation domain of Gcn4 bound to the mediator co-activator domain 1 of Gal11/med15

spinqualitylabelsall models 2lpb, 13 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2lpb is a 2 chain structure with sequence from Saccharomyces cerevisiae. This structure supersedes the now removed PDB entry 2ko4. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: ABE1, GAL11, MED15, RAR3, SDS4, SPT13, YOL051W (Saccharomyces cerevisiae), AAS3, ARG9, GCN4, YEL009C (Saccharomyces cerevisiae) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The structural basis for binding of the acidic transcription activator Gcn4 and one activator-binding domain of the Mediator subunit Gal11/Med15 was examined by NMR. Gal11 activator-binding domain 1 has a four-helix fold with a small shallow hydrophobic cleft at its center. In the bound complex, eight residues of Gcn4 adopt a helical conformation, allowing three Gcn4 aromatic/aliphatic residues to insert into the Gal11 cleft. The protein-protein interface is dynamic and surprisingly simple, involving only hydrophobic interactions. This allows Gcn4 to bind Gal11 in multiple conformations and orientations, an example of a "fuzzy" complex, where the Gcn4-Gal11 interface cannot be described by a single conformation. Gcn4 uses a similar mechanism to bind two other unrelated activator-binding domains. Functional studies in yeast show the importance of residues at the protein interface, define the minimal requirements for a functional activator, and suggest a mechanism by which activators bind to multiple unrelated targets. The acidic transcription activator Gcn4 binds the mediator subunit Gal11/Med15 using a simple protein interface forming a fuzzy complex.,Brzovic PS, Heikaus CC, Kisselev L, Vernon R, Herbig E, Pacheco D, Warfield L, Littlefield P, Baker D, Klevit RE, Hahn S Mol Cell. 2011 Dec 23;44(6):942-53. PMID:22195967 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Gcn4 Mediator

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