1xsp | pdb_00001xsp
Crystal Structure of human DNA polymerase lambda in complex with nicked DNA and pyrophosphate
| |||||||||||||
| 1xsp, resolution 2.20Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | NA and PPV | ||||||||||||
| Gene: | POLL (Homo sapiens) | ||||||||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
Pol lambda is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pol lambda representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pol lambda does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.
About this Structure
1XSP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A closed conformation for the Pol lambda catalytic cycle., Garcia-Diaz M, Bebenek K, Krahn JM, Kunkel TA, Pedersen LC, Nat Struct Mol Biol. 2005 Jan;12(1):97-8. Epub 2004 Dec 19. PMID:15608652
Page seeded by OCA on Thu Mar 20 15:14:03 2008
