1xt9 | pdb_00001xt9
Crystal Structure of Den1 in complex with Nedd8
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| 1xt9, resolution 2.20Å | |||||||||||||
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| Gene: | SENP8, FKSG8, PRSC2 (Homo sapiens), NEDD8 (Homo sapiens) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
The Nedd8 conjugation pathway is conserved from yeast to humans and is essential in many organisms. Nedd8 is conjugated to cullin proteins in a process that alters SCF E3 ubiquitin ligase activity, and it is presumed that Nedd8 deconjugation would reverse these effects. We now report the X-ray structures of the human Nedd8-specific protease, Den1, in a complex with the inhibitor Nedd8 aldehyde, thus revealing a model for the tetrahedral transition state intermediate generated during proteolysis. Although Den1 is closely related to the SUMO-specific protease family (Ulp/Senp family), structural analysis of the interface suggests determinants involved in Nedd8 selectivity by Den1 over other ubiquitin-like family members and suggests how the Ulp/Senp architecture has been modified to interact with different ubiquitin-like modifiers.
About this Structure
1XT9 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1., Reverter D, Wu K, Erdene TG, Pan ZQ, Wilkinson KD, Lima CD, J Mol Biol. 2005 Jan 7;345(1):141-51. PMID:15567417
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