2az3 | pdb_00002az3
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| 2az3, resolution 2.20Å | |||||||||||||
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| Ligands: | MG and CDP | ||||||||||||
| Gene: | ndk (Halobacterium salinarum) | ||||||||||||
| Activity: | Nucleoside-diphosphate kinase, with EC number 2.7.4.6 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum in complex with CDP
Overview
Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged enzyme, whereas the untagged form was used for co-crystallization with the nucleotide. Crosslinking under different salt and pH conditions revealed a stronger oligomerization tendency for the tagged protein at low and high salt concentrations. The influence of the His6-tag on the halophilic nature of the enzyme is discussed on the basis of the observed structural properties.
About this Structure
2AZ3 is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.
Reference
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum., Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, Oesterhelt D, FEBS Lett. 2005 Dec 5;579(29):6595-600. Epub 2005 Nov 9. PMID:16293253
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