4a8a | pdb_00004a8a

Asymmetric cryo-EM reconstruction of E. coli DegQ 12-mer in complex with lysozyme

spinqualitylabelsall models 4a8a, resolution 14.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4a8a is a 13 chain structure with sequence from Escherichia coli and Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 1kxx, 2ybi, 1t6v, 1vds, 1kir, 1yil, 1heo, 1ior, 1ljg, 1jis, 2w1m, 1uic, 1ykz, 1xgq, 2war, 1lj3, 1dpw, 8lyz, 2lyo, 1g7h, 1lks, 5lyt, 1sfb, 1ir7, 1xei, 1hel, 1ljf, 1f10, 1ljk, 6lyt, 1zmy, 2d91, 2xth, 1lze, 1aki, 1yik, 2d6b, 1lpi, 1ndg, 1flw, 4lyt, 1jit, 1lzn, 1ja2, 1wtn, 2fbb, 2lym, 1lz9, 1lse, 1lsm, 3lym, 7lyz, 1t3p, 2bly, 1b0d, 1her, 1ioq, 1nby, 1qio, 1xgp, 1ps5, 1gwd, 1v7t, 1h6m, 1dqj, 2a7d, 1z55, 2c8p, 1lsb, 1flq, 1jj1, 2ybl, 1yqv, 1hsx, 2cds, 1hf4, 1uib, 1ir9, 1rjc, 1ic4, 1ljh, 1xej, 1ja7, 1mel, 1ri8, 1c10, 1lyo, 2xjw, 1n4f, 2w1y, 1g7m, 1sf7, 1lsf, 1fn5, 2d4j, 1c08, 3lyz, 1bvk, 1uif, 1vau, 1lma, 1hc0, 1a2y, 4lzt, 1gxv, 1p2c, 1ic5, 1ua6, 1at5, 1vfb, 1vat, 1lj4, 1ja4, 4lym, 1flu, 1bwh, 2hfm, 1io5, 2bpu, 1lzb, 1lsc, 1w6z, 2ybn, 3lyo, 4lyo, 1kip, 1ic7, 1lzt, 2xbr, 1lys, 1bwj, 1e8l, 132l, 1sfg, 1kxw, 2x0a, 2c8o, 1g7l, 1sf4, 1yl1, 1h87, 3lyt, 1iot, 1dpx, 1v7s, 1ja6, 1ir8, 1uie, 1lji, 1bwi, 2iff, 1jj0, 1rfp, 1jiy, 1iee, 1xek, 1at6, 2ydg, 1mlc, 2b5z, 193l, 1lsz, 2ybm, 1sq2, 2ybh, 1vdq, 1lje, 1b2k, 2ybj, 9lyz, 1uia, 1hen, 1xfp, 1lsd, 2blx, 6lyz, 1nbz, 1lsg, 3hfm, 1ved, 1lyz, 1uuz, 2xbs, 2d4i, 1fdl, 1gxx, 1lzh, 1jj3, 1yky, 1kiq, 1heq, 1uih, 2lzh, 1kxy, 2w1l, 1g7j, 1bhz, 1wtm, 1hep, 1jtt, 1lza, 3zvq, 1jpo, 1j1p, 1ljj, 1f0w, 2w1x, 1lzg, 1lzc, 1rcm, 1uid, 1bgi, 1lzd, 1lcn, 1hew, 2vb1, 2aub, 1j1x, 1ios, 2cgi, 1uc0, 1azf, 4lyz, 1gpq, 2a6u, 2d4k, 1bvx, 1uig, 4a7d, 1qtk, 1lkr, 1hsw, 1jto, 5lyz, 3lzt, 1ndm, 1sf6, 2lyz, 1fly, 1yl0, 1j1o, 2lzt, 1lsy, 1uco, 5lym, 1lsa, 1hem, 1f3j, 2a7f, 194l, 1lz8, 1ykx, 1vdt, 1lsn, 1g7i, 1vdp Activity: Hydrolase, with EC number 3.4.21.107 Resources: FirstGlance, OCA, RCSB, PDBsum Function [DEGQ_ECOLI] DegQ could degrade transiently denatured and unfolded proteins which accumulate in the periplasm following stress conditions. DegQ is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for a beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable to be cleaved, thereby preventing non-specific proteolysis of folded proteins. DegQ can substitute for the periplasmic protease DegP. [LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus. Publication Abstract from PubMed The HtrA protein family combines chaperone and protease activities and is essential for protein quality control in many organisms. Whereas the mechanisms underlying the proteolytic function of HtrA proteins are well characterized, their chaperone activity remains poorly understood. Here we describe cryo-EM structures of Escherichia coli DegQ in its 12- and 24-mer states in complex with model substrates, providing a structural model of HtrA chaperone action. Up to six lysozyme substrates bind inside the DegQ 12-mer cage and are visualized in a close-to-native state. An asymmetric reconstruction reveals the binding of a well-ordered lysozyme to four DegQ protomers. DegQ PDZ domains are located adjacent to substrate density and their presence is required for chaperone activity. The substrate-interacting regions appear conserved in 12- and 24-mer cages, suggesting a common mechanism of chaperone function. Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ.,Malet H, Canellas F, Sawa J, Yan J, Thalassinos K, Ehrmann M, Clausen T, Saibil HR Nat Struct Mol Biol. 2012 Jan 15;19(2):152-7. doi: 10.1038/nsmb.2210. PMID:22245966 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Lysozyme 3D structures

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