2l4h | pdb_00002l4h
The Solution Structure of Calcium Bound CIB1
Structural highlights
Function[CIB1_HUMAN] May convert the inactive conformation of integrin alpha-IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation.[1] [2] [3] [4] [5] [6] Publication Abstract from PubMedThe Calcium and Integrin Binding protein 1 (CIB1) is a ubiquitous Ca(2+) binding protein and a specific binding partner for the platelet integrin alphaIIb cytoplasmic domain, which confers CIB1's key role in haemostasis. CIB1 is also known to be involved in apoptosis, embryogenesis and the DNA damage response. In this work, the solution structures of both Ca(2+)-CIB1 and Mg(2+)-CIB1 were determined using solution-state NMR spectroscopy. The methyl groups of Ile,Leu and Val were selectively protonated to compensate for the loss of protons due to deuteration. The solution structure of Ca(2+)-CIB1 possesses smaller opened EF-hands in its C-domain compared to available crystal structures. Ca(2+)-CIB1 and Mg(2+)-CIB1 have similar structures, but the N-lobe of Mg(2+)-CIB1 is slightly more opened than that of Ca(2+)-CIB1. Additional NMR experiments, such as chemical shift perturbation and methyl group solvent accessibility as measured by the surface probe TEMPOL were carried out to further characterize the structures of Ca(2+)-CIB1 and Mg(2+)-CIB1 as well as their interactions with the integrin alphaIIb cytoplasmic domain. NMR measurements of backbone amide proton slow motion (mus-ms) dynamics confirmed that the C-terminal helix of Ca(2+)-CIB1 is displaced upon alphaIIb binding. The EF-hand III of both Ca(2+)-CIB1 and Mg(2+)-CIB1 was identified to be directly involved in the interaction of CIB1 and alphaIIb. Together, these data illustrate that CIB1 behaves quite different from related EF-hand regulatory calcium-binding proteins, such as calmodulin or neuronal calcium sensor proteins. The solution structures of Ca2+- CIB1 and Mg2+-CIB1 and their interactions with the platelet integrin {alpha}IIb cytoplasmic domain.,Huang H, Ishida H, Yamniuk AP, Vogel HJ J Biol Chem. 2011 Mar 9. PMID:21388953[7] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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