2liy | pdb_00002liy

Plant peptide hormone regulating stomatal density

spinqualitylabelsall models 2liy, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2liy is a 1 chain structure with sequence from Arabidopsis thaliana. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: EPFL9 (Arabidopsis thaliana) Resources: FirstGlance, OCA, RCSB, PDBsum Function [EPFL9_ARATH] Controls stomatal patterning (By similarity). Publication Abstract from PubMed Stomatal development in plants is regulated by defensin-like secretory epidermal patterning factor (EPF) peptide hormones. Only one of these, stomagen, is a positive regulator, whereas EPF1, EPF2, and possibly others are negative regulators. Here we explore the structure-function relationships of EPFs, by integrating NMR and semi-in vitro stomagen experiments. We show that stomagen is composed of a loop and a scaffold containing three disulphide bonds. A mutant composed of the stomagen loop and the EPF2 scaffold positively regulates the stomatal density on Arabidopsis cotyledons. The reciprocal mutant composed of the EPF2 loop and the stomagen scaffold acts negatively. Deletion of the disulphide bond introduces unfolding and inactivity. Our results suggest that the loop confers the functional specificity of EPFs and that the scaffold is structurally required for their activity. This structural decomposition approach to elucidating the functional site could be adapted for the analysis of other cysteine-rich peptide families. The NMR structure of stomagen reveals the basis of stomatal density regulation by plant peptide hormones.,Ohki S, Takeuchi M, Mori M Nat Commun. 2011 Oct 25;2:512. doi: 10.1038/ncomms1520. PMID:22027592 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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