| Structural highlights
Publication Abstract from PubMed
Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-A resolution. This map, together with a 6.1-A map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model approximately 98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.,Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Soding J, Westhof E, Wilson DN, Beckmann R Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19748-53. Epub 2010 Oct 27. PMID:20980660[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Soding J, Westhof E, Wilson DN, Beckmann R. Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19748-53. Epub 2010 Oct 27. PMID:20980660 doi:10.1073/pnas.1009999107
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