3v0a | pdb_00003v0a

2.7 angstrom crystal structure of BoNT/Ai in complex with NTNHA

spinqualitylabelsall models 3v0a, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3v0a is a 3 chain structure with sequence from Clostridium botulinum and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, MES, SO4, ZN Related: 3v0b, 3v0c Gene: bont/a, boNT/A, bonta (Clostridium botulinum), ant, ntnh (Clostridium botulinum) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Botulinum neurotoxins (BoNTs) are highly poisonous substances that are also effective medicines. Accidental BoNT poisoning often occurs through ingestion of Clostridium botulinum-contaminated food. Here, we present the crystal structure of a BoNT in complex with a clostridial nontoxic nonhemagglutinin (NTNHA) protein at 2.7 angstroms. Biochemical and functional studies show that NTNHA provides large and multivalent binding interfaces to protect BoNT from gastrointestinal degradation. Moreover, the structure highlights key residues in BoNT that regulate complex assembly in a pH-dependent manner. Collectively, our findings define the molecular mechanisms by which NTNHA shields BoNT in the hostile gastrointestinal environment and releases it upon entry into the circulation. These results will assist in the design of small molecules for inhibiting oral BoNT intoxication and of delivery vehicles for oral administration of biologics. Botulinum neurotoxin is shielded by NTNHA in an interlocked complex.,Gu S, Rumpel S, Zhou J, Strotmeier J, Bigalke H, Perry K, Shoemaker CB, Rummel A, Jin R Science. 2012 Feb 24;335(6071):977-81. PMID:22363010 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody Botulinum neurotoxin

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