3tbk | pdb_00003tbk

Mouse RIG-I ATPase Domain

spinqualitylabelsall models 3tbk, resolution 2.14Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3tbk is a 1 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ANP, EDO NonStd Res: MSE Gene: Ddx58 (Mus musculus) Activity: RNA helicase, with EC number 3.6.4.13 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed RIG-I detects cytosolic viral dsRNA with 5' triphosphates (5'-ppp-dsRNA), thereby initiating an antiviral innate immune response. Here we report the crystal structure of superfamily 2 (SF2) ATPase domain of RIG-I in complex with a nucleotide analogue. RIG-I SF2 comprises two RecA-like domains 1A and 2A and a helical insertion domain 2B, which together form a 'C'-shaped structure. Domains 1A and 2A are maintained in a 'signal-off' state with an inactive ATP hydrolysis site by an intriguing helical arm. By mutational analysis, we show surface motifs that are critical for dsRNA-stimulated ATPase activity, indicating that dsRNA induces a structural movement that brings domains 1A and 2A/B together to form an active ATPase site. The structure also indicates that the regulatory domain is close to the end of the helical arm, where it is well positioned to recruit 5'-ppp-dsRNA to the SF2 domain. Overall, our results indicate that the activation of RIG-I occurs through an RNA- and ATP-driven structural switch in the SF2 domain. The RIG-I ATPase domain structure reveals insights into ATP-dependent antiviral signalling.,Civril F, Bennett M, Moldt M, Deimling T, Witte G, Schiesser S, Carell T, Hopfner KP EMBO Rep. 2011 Oct 7. doi: 10.1038/embor.2011.190. PMID:21979817 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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