3ub9 | pdb_00003ub9

Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with hydroxyurea bound

spinqualitylabelsall models 3ub9, resolution 1.42Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ub9 is a 2 chain structure with sequence from Helicobacter pylori ss1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, NHY, SO4 Related: 3ub6, 3ub7, 3ub8 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed pH sensing is crucial for survival of most organisms, yet the molecular basis of such sensing is poorly understood. Here, we present an atomic resolution structure of the periplasmic portion of the acid-sensing chemoreceptor, TlpB, from the gastric pathogen Helicobacter pylori. The structure reveals a universal signaling fold, a PAS domain, with a molecule of urea bound with high affinity. Through biophysical, biochemical, and in vivo mutagenesis studies, we show that urea and the urea-binding site residues play critical roles in the ability of H. pylori to sense acid. Our signaling model predicts that protonation events at Asp114, affected by changes in pH, dictate the stability of TlpB through urea binding. Structure and Proposed Mechanism for the pH-Sensing Helicobacter pylori Chemoreceptor TlpB.,Goers Sweeney E, Henderson JN, Goers J, Wreden C, Hicks KG, Foster JK, Parthasarathy R, Remington SJ, Guillemin K Structure. 2012 Jun 14. PMID:22705207 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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