4ads | pdb_00004ads

Crystal structure of plasmodial PLP synthase complex

spinqualitylabelsall models 4ads, resolution 3.61Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ads is a 12 chain structure with sequence from Plasmodium berghei and Plasmodium falciparum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PO4 Related: 4adt, 2abw, 4adu Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Biosynthesis of vitamins is fundamental to malaria parasites. Plasmodia synthesize the active form of vitamin B(6) (pyridoxal 5'-phosphate, PLP) using a PLP synthase complex. The EM analysis shown here reveals a random association pattern of up to 12 Pdx2 glutaminase subunits to the dodecameric Pdx1 core complex. Interestingly, Plasmodium falciparum PLP synthase organizes in fibers. The crystal structure shows differences in complex formation to bacterial orthologs as interface variations. Alternative positioning of an alpha helix distinguishes an open conformation from a closed state when the enzyme binds substrate. The pentose substrate is covalently attached through its C1 and forms a Schiff base with Lys84. Ammonia transfer between Pdx2 glutaminase and Pdx1 active sites is regulated by a transient tunnel. The mutagenesis analysis allows defining the requirement for conservation of critical methionines, whereas there is also plasticity in ammonia tunnel construction as seen from comparison across different species. Assembly of the Eukaryotic PLP-Synthase Complex from Plasmodium and Activation of the Pdx1 Enzyme.,Guedez G, Hipp K, Windeisen V, Derrer B, Gengenbacher M, Bottcher B, Sinning I, Kappes B, Tews I Structure. 2012 Jan 11;20(1):172-84. PMID:22244765 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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