4b02 | pdb_00004b02

The C-terminal Priming Domain is Strongly Associated with the Main Body of Bacteriophage phi6 RNA-Dependent RNA Polymerase

spinqualitylabelsall models 4b02, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4b02 is a 3 chain structure with sequence from Pseudomonas phage phi6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MN Related: 1hhs, 1hht, 1hi0, 1hi1, 1hi8, 1uvi, 1uvj, 1uvk, 1uvl, 1uvm, 1uvn, 1wac, 2jl9, 2jlf, 2jlg, 4a8f, 4a8k, 4a8m, 4a8o, 4a8q, 4a8s, 4a8w, 4a8y Activity: RNA-directed RNA polymerase, with EC number 2.7.7.48 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Double-stranded RNA viruses encode a single protein species containing RNA-dependent RNA polymerase (RdRP) motifs. This protein is responsible for RNA transcription and replication. The architecture of viral RdRPs resembles that of a cupped right hand with fingers, palm and thumb domains. Those using de novo initiation have a flexible structural elaboration that constitutes the priming platform. Here we investigate the properties of the C-terminal priming domain of bacteriophage varphi6 to get insights into the role of an extended loop connecting this domain to the main body of the polymerase. Proteolyzed varphi6 RdRP that possesses a nick in the hinge region of this loop was better suited for de novo initiation. The clipped C-terminus remained associated with the main body of the polymerase via the anchor helix. The structurally flexible hinge region appeared to be involved in the control of priming platform movement. Moreover, we detected abortive initiation products for a bacteriophage RdRP. The C-terminal priming domain is strongly associated with the main body of bacteriophage varphi6 RNA-dependent RNA polymerase.,Sarin LP, Wright S, Chen Q, Degerth LH, Stuart DI, Grimes JM, Bamford DH, Poranen MM Virology. 2012 Oct 10;432(1):184-93. Epub 2012 Jul 6. PMID:22770923 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase

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