4csf | pdb_00004csf

Structural insights into Toscana virus RNA encapsidation

spinqualitylabelsall models 4csf, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4csf is a 36 chain structure with sequence from Tos. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 4csg Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses. Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein.,Olal D, Dick A, Woods VL Jr, Liu T, Li S, Devignot S, Weber F, Saphire EO, Daumke O Nucleic Acids Res. 2014 Mar 31. PMID:24688060 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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