4fac | pdb_00004fac

Succinyl-CoA:acetate CoA-transferase (AarCH6) in complex with dethiaCoA, acetate, and a covalent acetylglutamyl anhydride adduct

spinqualitylabelsall models 4fac, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4fac is a 2 chain structure with sequence from Acetobacter aceti. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 0T1, ACE, ACT, CL Related: 4eu3, 4eu4, 4eu5, 4eu6, 4eu7, 4eu8, 4eu9, 4eua, 4eub, 4euc, 4eud Gene: aarC (Acetobacter aceti) Activity: Succinyl-CoA:acetate CoA-transferase, with EC number 2.8.3.18 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Microbes tailor macromolecules and metabolism to overcome specific environmental challenges. Acetic acid bacteria perform the aerobic oxidation of ethanol to acetic acid and are generally resistant to high levels of these two membrane-permeable poisons. The citric acid cycle (CAC) is linked to acetic acid resistance in Acetobacter aceti by several observations, among them the oxidation of acetate to CO2 by highly resistant acetic acid bacteria and the previously unexplained role of A. aceti citrate synthase (AarA) in acetic acid resistance at a low pH. Here we assign specific biochemical roles to the other components of the A. aceti strain 1023 aarABC region. AarC is succinyl-coenzyme A (CoA):acetate CoA-transferase, which replaces succinyl-CoA synthetase in a variant CAC. This new bypass appears to reduce metabolic demand for free CoA, reliance upon nucleotide pools, and the likely effect of variable cytoplasmic pH upon CAC flux. The putative aarB gene is reassigned to SixA, a known activator of CAC flux. Carbon overflow pathways are triggered in many bacteria during metabolic limitation, which typically leads to the production and diffusive loss of acetate. Since acetate overflow is not feasible for A. aceti, a CO(2) loss strategy that allows acetic acid removal without substrate-level (de)phosphorylation may instead be employed. All three aar genes, therefore, support flux through a complete but unorthodox CAC that is needed to lower cytoplasmic acetate levels. A specialized citric acid cycle requiring succinyl-coenzyme A (CoA):acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter aceti.,Mullins EA, Francois JA, Kappock TJ J Bacteriol. 2008 Jul;190(14):4933-40. Epub 2008 May 23. PMID:18502856 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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