4fyf | pdb_00004fyf

Structural basis for substrate recognition by a novel Legionella phosphoinositide phosphatase

spinqualitylabelsall models 4fyf, resolution 2.42Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4fyf is a 1 chain structure with sequence from Legionella pneumophila subsp. pneumophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: HG, PO4 Related: 4fye Gene: lpg2584, sidF (Legionella pneumophila subsp. pneumophila) Activity: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase, with EC number 3.1.3.67 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Legionella pneumophila is an opportunistic intracellular pathogen that causes sporadic and epidemic cases of Legionnaires' disease. Emerging data suggest that Legionella infection involves the subversion of host phosphoinositide (PI) metabolism. However, how this bacterium actively manipulates PI lipids to benefit its infection is still an enigma. Here, we report that the L. pneumophila virulence factor SidF is a phosphatidylinositol polyphosphate 3-phosphatase that specifically hydrolyzes the D3 phosphate of PI(3,4)P(2) and PI(3,4,5)P(3). This activity is necessary for anchoring of PI(4)P-binding effectors to bacterial phagosomes. Crystal structures of SidF and its complex with its substrate PI(3,4)P(2) reveal striking conformational rearrangement of residues at the catalytic site to form a cationic pocket that specifically accommodates the D4 phosphate group of the substrate. Thus, our findings unveil a unique Legionella PI phosphatase essential for the establishment of lipid identity of bacterial phagosomes. Structural basis for substrate recognition by a unique Legionella phosphoinositide phosphatase.,Hsu F, Zhu W, Brennan L, Tao L, Luo ZQ, Mao Y Proc Natl Acad Sci U S A. 2012 Aug 7. PMID:22872863 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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