4k59 | pdb_00004k59

Crystal Structure of Pseudomonas Aeruginosa RsmF

spinqualitylabelsall models 4k59, resolution 2.22Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4k59 is a 1 chain structure with sequence from Pseudomonas aeruginosa ucbpp-pa14. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: PA14_68470 (Pseudomonas aeruginosa UCBPP-PA14) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Members of the CsrA family of prokaryotic mRNA-binding proteins alter the translation and/or stability of transcripts needed for numerous global physiological processes. The previously described CsrA family member in Pseudomonas aeruginosa (RsmA) plays a central role in determining infection modality by reciprocally regulating processes associated with acute (type III secretion and motility) and chronic (type VI secretion and biofilm formation) infection. Here we describe a second, structurally distinct RsmA homolog in P. aeruginosa (RsmF) that has an overlapping yet unique regulatory role. RsmF deviates from the canonical 5 beta-strand and carboxyl-terminal alpha-helix topology of all other CsrA proteins by having the alpha-helix internally positioned. Despite striking changes in topology, RsmF adopts a tertiary structure similar to other CsrA family members and binds a subset of RsmA mRNA targets, suggesting that RsmF activity is mediated through a conserved mechanism of RNA recognition. Whereas deletion of rsmF alone had little effect on RsmA-regulated processes, strains lacking both rsmA and rsmF exhibited enhanced RsmA phenotypes for markers of both type III and type VI secretion systems. In addition, simultaneous deletion of rsmA and rsmF resulted in superior biofilm formation relative to the wild-type or rsmA strains. We show that RsmF translation is derepressed in an rsmA mutant and demonstrate that RsmA specifically binds to rsmF mRNA in vitro, creating a global hierarchical regulatory cascade that operates at the posttranscriptional level. An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa.,Marden JN, Diaz MR, Walton WG, Gode CJ, Betts L, Urbanowski ML, Redinbo MR, Yahr TL, Wolfgang MC Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15055-60. doi:, 10.1073/pnas.1307217110. Epub 2013 Aug 26. PMID:23980177 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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