Structural highlights
Publication Abstract from PubMedThe structure of BC0361, a polysaccharide deacetylase from Bacillus cereus, has been determined using an unconventional molecular-replacement procedure. Tens of putative models of the C-terminal domain of the protein were constructed using a multitude of homology-modelling algorithms, and these were tested for the presence of signal in molecular-replacement calculations. Of these, only the model calculated by the SAM-T08 server gave a consistent and convincing solution, but the resulting model was too inaccurate to allow phase determination to proceed to completion. The application of slow-cooling torsion-angle simulated annealing (started from a very high temperature) drastically improved this initial model to the point of allowing phasing through cycles of model building and refinement to be initiated. The structure of the protein is presented with emphasis on the presence of a C(alpha)-modified proline at its active site, which was modelled as an alpha-hydroxy-L-proline. Structure determination through homology modelling and torsion-angle simulated annealing: application to a polysaccharide deacetylase from Bacillus cereus.,Fadouloglou VE, Kapanidou M, Agiomirgianaki A, Arnaouteli S, Bouriotis V, Glykos NM, Kokkinidis M Acta Crystallogr D Biol Crystallogr. 2013 Feb;69(Pt 2):276-83. doi:, 10.1107/S0907444912045829. Epub 2013 Jan 19. PMID:23385463[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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