4mb3 | pdb_00004mb3

Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella marina

spinqualitylabelsall models 4mb3, resolution 1.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4mb3 is a 1 chain structure with sequence from "spirillum_marinum"_russell_1891 "spirillum marinum" russell 1891. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, GLY, IMD, MPD, NA Related: 4hmc, 4hmd, 4hme, 4mb4, 4mb5 Gene: chi60 ("Spirillum marinum" Russell 1891) Activity: Chitinase, with EC number 3.2.1.14 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact. Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.,Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):676-84. doi:, 10.1107/S1399004713032264. Epub 2014 Feb 15. PMID:24598737 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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