4c8z | pdb_00004c8z

Cas6 (TTHA0078) product complex

spinqualitylabelsall models 4c8z, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4c8z is a 3 chain structure with sequence from Thet8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: K, SO4 NonStd Res: 23G Related: 4c8y, 4c97, 4c98, 4c9d Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed In many bacteria and archaea, small RNAs derived from clustered regularly interspaced short palindromic repeats (CRISPRs) associate with CRISPR-associated (Cas) proteins to target foreign DNA for destruction. In Type I and III CRISPR/Cas systems, the Cas6 family of endoribonucleases generates functional CRISPR-derived RNAs by site-specific cleavage of repeat sequences in precursor transcripts. CRISPR repeats differ widely in both sequence and structure, with varying propensity to form hairpin folds immediately preceding the cleavage site. To investigate the evolution of distinct mechanisms for the recognition of diverse CRISPR repeats by Cas6 enzymes, we determined crystal structures of two Thermus thermophilus Cas6 enzymes both alone and bound to substrate and product RNAs. These structures show how the scaffold common to all Cas6 endonucleases has evolved two binding sites with distinct modes of RNA recognition: one specific for a hairpin fold and the other for a single-stranded 5'-terminal segment preceding the hairpin. These findings explain how divergent Cas6 enzymes have emerged to mediate highly selective pre-CRISPR-derived RNA processing across diverse CRISPR systems. Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases.,Niewoehner O, Jinek M, Doudna JA Nucleic Acids Res. 2013 Oct 22. PMID:24150936 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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