4ccw | pdb_00004ccw

Crystal structure of naproxen esterase (carboxylesterase NP) from Bacillus subtilis

spinqualitylabelsall models 4ccw, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ccw is a 1 chain structure with sequence from "bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: VKC Related: 4ccy Activity: Carboxylesterase, with EC number 3.1.1.1 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Naproxen esterase (NP) from Bacillus subtilis Thai I-8 is a carboxylesterase that catalyzes the enantioselective hydrolysis of naproxenmethylester to produce S-naproxen (E>200). It is a homolog of CesA (98% sequence identity) and CesB (64% identity), both produced by B. subtilis strain 168. CesB can be used for the enantioselective hydrolysis of 1,2-O-isopropylideneglycerol (solketal) esters (E>200 for IPG-caprylate). Crystal structures of NP and CesB, determined to a resolution of 1.75A and 2.04A, respectively, showed that both proteins have a canonical alpha/beta hydrolase fold with an extra N-terminal helix stabilizing the cap subdomain. The active site in both enzymes is located in a deep hydrophobic groove and includes the catalytic triad residues Ser130, His274, and Glu245. A product analog, presumably 2-(2-hydroxyethoxy)acetic acid, was bound in the NP active site. The enzymes have different enantioselectivities, which previously were shown to result from only a few amino acid substitutions in the cap domain. Modeling of a substrate in the active site of NP allowed explaining the different enantioselectivities. In addition, Ala156 may be a determinant of enantioselectivity as well, since its side chain appears to interfere with the binding of certain R-enantiomers in the active site of NP. However, the exchange route for substrate and product between the active site and the solvent is not obvious from the structures. Flexibility of the cap domain might facilitate such exchange. Interestingly, both carboxylesterases show higher structural similarity to meta-cleavage compound (MCP) hydrolases than to other alpha/beta hydrolase fold esterases. Crystal structures of two Bacillus carboxylesterases with different enantioselectivities.,Rozeboom HJ, Godinho LF, Nardini M, Quax WJ, Dijkstra BW Biochim Biophys Acta. 2014 Mar;1844(3):567-75. doi: 10.1016/j.bbapap.2014.01.003., Epub 2014 Jan 11. PMID:24418394 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Carboxylesterase

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