2chk | pdb_00002chk

NMR STRUCTURE OF TLLLLT QUADRUPLEX

spinqualitylabelsall models 2chk, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2chk is a 4 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: LCG Related: 2chj Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars. In particular, a distinct change in the sugar-phosphate backbone is observed at the G2pL3 and L2pL3 base steps and sequence dependent changes in the twist between tetrads are also seen. Both the LNA modified quadruplexes have raised thermostability as compared to the DNA quadruplex. The quadruplex-forming capability of d(TGLGLT) is of particular interest as it expands the design flexibility for stable parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with DNA or other modified nucleic acids. As such, LNA-based quadruplexes can be decorated by a variety of chemical modifications. Such LNA quadruplex scaffolds might find applications in the developing field of nanobiotechnology. NMR solution structures of LNA (locked nucleic acid) modified quadruplexes.,Nielsen JT, Arar K, Petersen M Nucleic Acids Res. 2006 Apr 13;34(7):2006-14. Print 2006. PMID:16614450 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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