3bzn | pdb_00003bzn

Crystal Structure of Open form of Menaquinone-Specific Isochorismate Synthase, MenF

spinqualitylabelsall models 3bzn, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3bzn is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, SO4 Related: 3bzm Gene: menF, yfbA (Escherichia coli) Activity: Isochorismate synthase, with EC number 5.4.4.2 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The electron carrier menaquinone is one of many important bacterial metabolites that are derived from the key intermediate chorismic acid. MenF, the first enzyme in the menaquinone pathway, catalyzes the isomerization of chorismate to isochorismate. Here, an improved structure of MenF in a new crystal form is presented. The structure, solved at 2.0 angstroms resolution in complex with magnesium, reveals a well defined closed active site. Existing evidence suggests that the mechanism of the reaction catalyzed by MenF involves nucleophilic attack of a water molecule on the chorismate ring. The structure reveals a well defined water molecule located in an appropriate position for activation by Lys190 and attack on the substrate. Structure of isochorismate synthase in complex with magnesium.,Parsons JF, Shi KM, Ladner JE Acta Crystallogr D Biol Crystallogr. 2008 May;64(Pt 5):607-10. Epub 2008, Apr 19. PMID:18453696 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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