4ur0 | pdb_00004ur0

Crystal structure of the PCE reductive dehalogenase from S. multivorans in complex with trichloroethene

spinqualitylabelsall models 4ur0, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ur0 is a 2 chain structure with sequence from Sulfurospirillum multivorans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BEN, BVQ, GOL, SF4, TCV Related: 4uqu, 4ur1, 4ur2, 4ur3 Activity: Tetrachloroethene reductive dehalogenase, with EC number 1.97.1.8 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Organohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region. Structural basis for organohalide respiration.,Bommer M, Kunze C, Fesseler J, Schubert T, Diekert G, Dobbek H Science. 2014 Oct 2. pii: 1258118. PMID:25278505 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA