3jwb | pdb_00003jwb

Crystal structure of L-methionine gamma-lyase from Citrobacter freundii with norleucine

spinqualitylabelsall models 3jwb, resolution 1.63Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3jwb is a 1 chain structure with sequence from "bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NLE, PEG NonStd Res: CSO, LLP Related: 2rfv, 1y4i, 2o7c, 1ukj, 1e5e, 3jw9, 3jwa Gene: megL ("Bacterium freundii" Braak 1928) Activity: Methionine gamma-lyase, with EC number 4.4.1.11 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Crystal structures of Citrobacter freundii methionine gamma-lyase complexes with the substrates of gamma- (L-1-amino-3-methylthiopropylphosphinic acid) and beta- (S-ethyl-L-cysteine) elimination reactions and the competitive inhibitor L-norleucine have been determined at 1.45, 1.8, and 1.63 A resolution, respectively. All three amino acids occupy the active site of the enzyme but do not form a covalent bond with pyridoxal 5'-phosphate. Hydrophobic interactions between the active site residues and the side groups of the substrates and the inhibitor are supposed to cause noncovalent binding. Arg374 and Ser339 are involved in the binding of carboxyl groups of the substrates and the inhibitor. The hydroxyl of Tyr113 is a potential acceptor of a proton from the amino groups of the amino acids. Three-dimensional structures of noncovalent complexes of Citrobacter freundii methionine gamma-lyase with substrates.,Revtovich SV, Morozova EA, Khurs EN, Zakomirdina LN, Nikulin AD, Demidkina TV, Khomutov RM Biochemistry (Mosc). 2011 May;76(5):564-70. doi: 10.1134/S0006297911050063. PMID:21639836 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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