2qmo | pdb_00002qmo
Crystal structure of dethiobiotin synthetase (bioD) from Helicobacter pylori
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| 2qmo, resolution 1.47Å | |||||||||||||
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| Ligands: | CL | ||||||||||||
| Gene: | bioD, HP_0029 (Helicobacter pylori 26695) | ||||||||||||
| Activity: | Dethiobiotin synthase, with EC number 6.3.3.3 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2QMO is a Single protein structure of sequence from Helicobacter pylori 26695. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Thu Mar 20 18:27:24 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Dethiobiotin synthase
- Helicobacter pylori 26695
- Single protein
- Chruszcz, M.
- Cuff, M.
- Cymborowski, M.
- Edwards, A.
- Joachimiak, A.
- MCSG, Midwest Center for Structural Genomics.
- Minor, W.
- Savchenko, A.
- Xu, X.
- Zheng, H.
- CL
- Atp-binding
- Biotin biosynthesis
- Dethiobiotin synthetase
- Ligase
- Magnesium
- Mcsg
- Midwest center for structural genomic
- Nucleotide-binding
- Protein structure initiative
- Psi-2
- Structural genomic
