4u7v | pdb_00004u7v

Structure of wild-type HIV protease in complex with degraded photosensitive inhibitor

spinqualitylabelsall models 4u7v, resolution 1.38Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4u7v is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 3EN, BME Related: 4u7q Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed HIV protease (PR) is required for proteolytic maturation in the late phase of HIV replication and represents a prime therapeutic target. The regulation and kinetics of viral polyprotein processing and maturation are currently not understood in detail. Here we design, synthesize, validate and apply a potent, photodegradable HIV PR inhibitor to achieve synchronized induction of proteolysis. The compound exhibits subnanomolar inhibition in vitro. Its photolabile moiety is released on light irradiation, reducing the inhibitory potential by 4 orders of magnitude. We determine the structure of the PR-inhibitor complex, analyze its photolytic products, and show that the enzymatic activity of inhibited PR can be fully restored on inhibitor photolysis. We also demonstrate that proteolysis of immature HIV particles produced in the presence of the inhibitor can be rapidly triggered by light enabling thus to analyze the timing, regulation and spatial requirements of viral processing in real time. Triggering HIV polyprotein processing by light using rapid photodegradation of a tight-binding protease inhibitor.,Schimer J, Pavova M, Anders M, Pachl P, Sacha P, Cigler P, Weber J, Majer P, Rezacova P, Krausslich HG, Muller B, Konvalinka J Nat Commun. 2015 Mar 9;6:6461. doi: 10.1038/ncomms7461. PMID:25751579 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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