2rcu | pdb_00002rcu

File:2rcu.gif

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2rcu, resolution 1.78Å
Ligands:	BOG and BUJ
Gene:	Cpt2, Cpt-2 (Rattus norvegicus)
Activity:	Carnitine O-palmitoyltransferase, with EC number 2.3.1.21
Coordinates:	save as pdb, mmCIF, xml

Crystal structure of rat carnitine palmitoyltransferase 2 in complex with r-3-(hexadecanoylamino)-4-(trimethylazaniumyl)butanoate

Overview

The mitochondrial membrane-associated carnitine palmitoyltransferase system is a validated target for the treatment of type 2 diabetes mellitus. To further facilitate structure-based drug discovery, we determined the crystal structure of rat CPT-2 (rCPT-2) in complex with the substrate analogue palmitoyl-aminocarnitine at 1.8A resolution. Biochemical analyses revealed a strong effect of this compound on rCPT-2 activity and stability. Using a computational approach we examined the membrane association of rCPT-2. The protein interacts with the membrane as a functional monomer and the calculations confirm the presence of a membrane association domain that consists of layers of hydrophobic and positively charged residues.

About this Structure

2RCU is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Carnitine palmitoyltransferase 2: analysis of membrane association and complex structure with a substrate analog., Rufer AC, Lomize A, Benz J, Chomienne O, Thoma R, Hennig M, FEBS Lett. 2007 Jul 10;581(17):3247-52. Epub 2007 Jun 8. PMID:17585909

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