4rlb | pdb_00004rlb

Crystal structure of Acinetobacter baumannii CarO2

spinqualitylabelsall models 4rlb, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4rlb is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 4fuv, 4rl9, 4rlc Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Outer membrane (OM) beta-barrel proteins composed of 12-18 beta-strands mediate cellular entry of small molecules in Gram-negative bacteria. Small OM proteins with barrels of 10 strands or less are not known to transport small molecules. CarO (carbapenem-associated outer membrane protein) from Acinetobacter baumannii is a small OM protein that has been implicated in the uptake of ornithine and carbapenem antibiotics. Here we report crystal structures of three isoforms of CarO. The structures are very similar and show a monomeric eight-stranded barrel lacking an open channel. CarO has a substantial extracellular domain resembling a glove that contains all the divergent residues between the different isoforms. Liposome swelling experiments demonstrate that full-length CarO and a "loop-less" truncation mutant mediate small-molecule uptake at low levels but that they are unlikely to mediate passage of carbapenem antibiotics. These results are confirmed by biased molecular dynamics simulations that allowed us to quantitatively model the transport of selected small molecules. Small-Molecule Transport by CarO, an Abundant Eight-Stranded beta-Barrel Outer Membrane Protein from Acinetobacterbaumannii.,Zahn M, D'Agostino T, Eren E, Basle A, Ceccarelli M, van den Berg B J Mol Biol. 2015 Apr 3. pii: S0022-2836(15)00201-6. doi:, 10.1016/j.jmb.2015.03.016. PMID:25846137 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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