3bd9 | pdb_00003bd9
human 3-O-sulfotransferase isoform 5 with bound PAP
| |||||||||||||
| 3bd9, resolution 2.30Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sites: | AC1 | ||||||||||||
| Ligands: | A3P | ||||||||||||
| Gene: | HS3ST5, 3OST5, HS3OST5 (Homo sapiens) | ||||||||||||
| Activity: | [Heparan_sulfate-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number 2.8.2.23 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
The biosynthesis of heparan sulfate (HS) involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations of these residues altered the substrate specificities. Our results demonstrate the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions.
About this Structure
3BD9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Engineering sulfotransferases to modify heparan sulfate., Xu D, Moon AF, Song D, Pedersen LC, Liu J, Nat Chem Biol. 2008 Mar;4(3):200-2. Epub 2008 Jan 27. PMID:18223645 [[Category: [Heparan sulfate]-glucosamine 3-sulfotransferase 1]]
Page seeded by OCA on Thu Mar 20 18:58:04 2008
