4xz7 | pdb_00004xz7

Crystal structure of a TGase

spinqualitylabelsall models 4xz7, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4xz7 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Streptococcus suis serotype 2 (S. suis 2, Ss2) is an important swine and human zoonotic pathogen. In the present study, we identified a novel secreted immunogenic protein, SsTGase, contained a highly conserved eukaryotic-like transglutaminase (TGase) domain at the N terminus. We found that inactivation of SsTGase significantly reduced the virulence of Ss2 in a pig infection model and impaired its anti-phagocytosis in human blood. We further solved the crystal structure of the N-terminal portion of the protein in homodimer form at 2.1 A. Structure based mutagenesis and biochemical studies suggested that disruption of the homodimer directly resulted in the loss of its TGase activity and anti-phagocytic ability. Characterization of SsTGase as a novel virulence factor of Ss2 by acting as a TGase would be beneficial for developing new therapeutic agents against Ss2 infection. Functional and structural characterization of the anti-phagocytic properties of a novel transglutaminase from Streptococcus suis.,Yu J, Pian Y, Ge J, Guo J, Zheng Y, Jiang H, Hao H, Yuan Y, Jiang Y, Yang M J Biol Chem. 2015 Jun 17. pii: jbc.M115.643338. PMID:26085092 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA