4zk7 | pdb_00004zk7

Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31

spinqualitylabelsall models 4zk7, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4zk7 is a 24 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, RCSB, PDBsum Function [CUTA_THET8] Involved in resistance toward heavy metals (By similarity). Publication Abstract from PubMed We recently reported the development of a computational method for the design of co-assembling, multi-component protein nanomaterials. While four such materials were validated at high-resolution by X-ray crystallography, low yield of soluble protein prevented X-ray structure determination of a fifth designed material, T33-09. Here we report the design and crystal structure of T33-31, a variant of T33-09 with improved soluble yield resulting from redesign efforts focused on mutating solvent-exposed side chains to charged amino acids. The structure is found to match the computational design model with atomic-level accuracy, providing further validation of the design approach and demonstrating a simple and potentially general means of improving the yield of designed protein nanomaterials. This article is protected by copyright. All rights reserved. Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.,Bale JB, Park RU, Liu Y, Gonen S, Gonen T, Cascio D, King NP, Yeates TO, Baker D Protein Sci. 2015 Jul 15. doi: 10.1002/pro.2748. PMID:26174163 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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