1gsp | pdb_00001gsp
| |||||||||||||
| 1gsp, resolution 2.20Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sites: | BI1, CAL and CAT | ||||||||||||
| Ligands: | CA and SGP | ||||||||||||
| Activity: | Ribonuclease T(1), with EC number 3.1.27.3 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS, 1 DAY
Overview
Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.
About this Structure
1GSP is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.
Reference
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218
Page seeded by OCA on Sun Mar 23 11:59:01 2008
